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KMID : 0613820170270060640
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Journal of Life Science
2017 Volume.27 No. 6 p.640 ~ p.645
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Anti-oxidant and Anti-inflammatory Properties of Clerodendrum trichotomum Leaf Extracts
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Kim Ji-Hye
Song Ha-Na
Ko Hee-Chul
Lee Ju-Yeop
Jang Mi-Gyeong
Kim Se-Jae
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Abstract
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Clerodendrum trichotomum (CT) leaves and stems have been used in folk medicine for their anti-hypertension, arthritis, rheumatism, and anti-inflammatory properties. This study was performed to evaluate the potential of CT as an anti-oxidant and anti-inflammatory agent. CT leaves were extracted using 70% ethanol (EtOH). Then, using this extract, a hexane, chloroform (CHCl3), ethyl acetate (EtOAc), and n-butanol (BuOH) fraction was prepared. The polyphenol contents were higher in the EtOAc fraction (78.08 μg/mg) and BuOH fraction (77.54 μg/mg) compared to the other fractions. Also, these two fractions exhibited strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activities. Xanthine oxidase inhibitory activities were higher in the CHCl3 fraction (IC50 = 4.43 μg/ml) and EtOAc fraction (IC50 = 5.69 μg/ml). Moreover, the EtOAc fraction effectively inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)- stimulated RAW 264.7 cells (IC50 = 18.87 μg/ml). Thus, we investigated the effects of the EtOAc fraction on the expression of pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 cells. The treatment of the EtOAc fraction (100 μg/ml) effectively decreased the levels of the tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6), and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). These results suggest the potential for CT extract and fractions as promising anti-oxidant and anti-inflammatory agents.
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KEYWORD
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Anti-inflammatory, anti-oxidant, Clerodendrum trichotomum, nitric oxide, polyphenol
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